Lactic acid bacteria LFK

LFK is a lactic acid bacterial material whose active components are extracted from Enterococcus faecalis FK-23 by enzyme digestion. Patents have been granted on LFK.

Lactic acid cocci, Enterococcus faecalis - LFK
LFK (lactic acid bacterial FK-23 extracts)

1. Trait

LFK is lactic acid bacterial extracts that is derived from enzymatic digestion of pure-cultivated Enterococcus faecalis FK-23. LFK as a food material contains over 9.0% of RNA (ribonucleic acid). Any extending agents are not included in this material (Egg-derived components are used as a part of raw materials).

2. Quality Standards

Standard item Standard value Test method
Dry weight percentage ≦ 5.0% Atomospheric heat dry
RNA (Nucleic acid) ≧ 9.0% Orcinol method
Heavy metal (Pb) ≦ 20ppm Colorimetric method with sodium sulfate
General bacteria count ≦ 3000 cells/g Standard agar plate culture method
Coliform Negative BGLB method
Fungi count ≦ 300 cells/g Petrifilm method

3. Examples of Food Labels

Lactic acid bacteria, Lactic acid bacterial powder, Heat-treated lactic acid bacteria, Lactic acid bacterial extracts, etc.
Notice: based on the Food Sanitation Law, it needs to specify the allergenic ingredient (egg).

Product Trait

1. About Enterococcus faecalis

Enterococcus faecalis is a regular component of the intestinal flora in healthy young adults. It is known that Enterococcus faecalis is deeply involved in maintaining human health.

Enterococcus faecalis

2. About LFK

LFK is enzyme-digested extracts from Enterococcus faecalis FK-23 that was screened at Nichinichi pharmaceutical Co., Ltd. For efficient effect on human body, LFK is processed to extract active components from inside bacterial cells.

3. Function of LFK

Active components in bacterial cells control functions in human body. Various values, which are not observed in live bacteria, are expected in LFK.

Patent [1] Anti-hypertensive effect Work on the blood vessel wall and dilate the blood vessel
Patent [2] Anti-allergic effect Improve immediate allergies such as hay fever and asthmatic symptoms
Patent [3] Cosmetic effect Improve brandy and pimpled face, and whiten the skin (Anti-pigmentation)
  [4] Calming effect of intestinal disorders Increase the number of probiotic bacteria in intestinal tract and maintain good intestinal condition
  [5] Combined effect with kampo medicine Enhance the effect of kampo medicine by the combined use

4. Feature of LFK

  • Contain abundant bacterial RNA (over 9.0%)
  • Original treating method (Granted patent)
  • Stable physicochemically
  • Stable preservation (Quality guarantee for two years)
  • Light colored powder
  • Combined use with medicines is possible

5. Safety of LFK

  • In the acute and subacute toxicity test using mice at high dose of LFK (2,000 mg/kg), no abnormality was observed. For human, usual intake is about 20–50 mg/kg.
  • In the chronic toxicity test using rats, no abnormality was observed.
  • In clinical test in healthy adults (intake of 1 g LFK/day for three months), no abnormality was observed on all general analyses such as blood pressure, biochemical test of blood, questionnaire and so on.
  • Enterococcus faecalis FK-23 is confirmed to be safe probiotic bacteria because of the absence of multidrug resistance genes (VRE; vanC-1, vanC-2, and vanC-3).

6. Application of LFK to health foods and the references

Research on LFK was published to academic journals, presented to academic societies, and granted patents. LFK is able to be utilized for various purposes as health food materials not only for human but also for pets, as described below.

  • Patent No. 3040744, Anti-allergic agent and its manufacturing process
  • Shimada et al., The 59th Annual meeting of Japanese Society of Allergology (2009)
  • Shimada et al., Japanese Society for lactic acid bacteria meeting (2009)
  • Shimada et al., J Investg Allergol Clin Immunol, 17, p70 (2007)
  • Shimada et al., Progress in medicine, 26, p1815 (2006)
  • Shimada et al., Allergology International, 54, p345 (2005)
  • Shimada et al., Clin Exp Allergy, 34, p1784 (2004)
Combination with kampo medicine
  • Shimada et al., Asian Pacific J Aller Immunol, 28, p59 (2010)
  • Kondoh et al., Annual meeting of medical and pharmaceutical society for WAKAN-YAKU (2008)
Cosmetic effect
  • Patent No. 2944662, Therapeutic agent on rosacea
  • Patent No. 4326905, Anti-pigmentation agent
  • Kobayashi et al., Annual meeting of Japanese Society for complementary and alternative medicine (2007)
  • Furuno et al., Fragrance Journal, 9, p85 (2005)
  • Sugai et al., Pharmacometrics, 63, p1 (2002)
  • Sugai et al., Pharmacometrics, 60, p33 (2001)
  • Patent No. 2889481, Anti-hypertensive agent
  • Patent No. 2889491, Anti-hypertensive agent
  • Motonaga et al., Annual meeting Japan Society for Bioscience, Biotechnology, and Agrochemistry (2004)
  • Shimada et al., Annual meeting Japan Society for Bioscience, Biotechnology, and Agrochemistry (1997)